| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pZeoSV2 (+) |
| 质粒类型: | 哺乳动物细胞载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶 |
| 启动子: | SV40 |
| 载体大小: | 3515 dp |
| 5' 测序引物及序列: | T7/SV40pro-F |
| 3' 测序引物及序列: | BGH rev |
| 载体标签: | -- |
| 载体抗性: | 博来霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | 非病毒 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体图谱.png)
载体简介
载体序列
LOCUS pZeoSV2(+) 3515 bp ds-DNA circular SYN 24-OCT-2017
DEFINITION Constitutive mammalian expression vector with the SV40 promoter and
a Zeocin(TM) resistance marker. See also pZeoSV2(-).
ACCESSION .
VERSION .
KEYWORDS pZeoSV2(+)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3515)
AUTHORS Thermo Fisher (Invitrogen)
TITLE Direct Submission
JOURNAL Exported Saturday, December 7, 2019 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3515
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
promoter 22..351
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 202..337
/note="SV40 ori"
/note="SV40 origin of replication"
promoter 401..419
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 433..548
/note="MCS"
/note="multiple cloning site"
polyA_signal 566..790
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin complement(873..1461)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
rep_origin 1816..2271
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
enhancer 2307..2610
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 2611..2814
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 2932..2979
/note="EM7 promoter"
/note="synthetic bacterial promoter "
CDS 2998..3372
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/note="BleoR"
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 3386..3507
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
ORIGIN
1 ggatcgatcc ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
61 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
121 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
181 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
241 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
301 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctctct
361 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
421 agacccaagc tggctagcgt ttaaacttaa gcttggtacc gagctcggat ccactagtcc
481 agtgtggtgg aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc
541 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
601 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
661 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
721 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
781 ggctctatgg cttctgaggc ggaaagaacc agcatgtgag caaaaggcca gcaaaaggcc
841 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
901 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
961 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
1021 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
1081 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
1141 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
1201 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
1261 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
1321 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
1381 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
1441 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
1501 tggaacgaaa actcacgtta agggattttg gtcatgacat taacctataa aaataggcgt
1561 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
1621 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
1681 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
1741 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
1801 aaataccgca tcaggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
1861 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
1921 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
1981 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
2041 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
2101 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
2161 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
2221 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt tccattcgcc
2281 attcaggctg aactagatct agagtccgtt acataactta cggtaaatgg cccgcctggc
2341 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
2401 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
2461 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
2521 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
2581 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
2641 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
2701 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
2761 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
2821 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
2881 cgggaccgat ccagcctccg cggccgggaa cggtgcattg gaacggaccg tgttgacaat
2941 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
3001 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
3061 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
3121 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
3181 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
3241 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
3301 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
3361 gagcaggact gacactcgac ctcgaaactt gtttattgca gcttataatg gttacaaata
3421 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
3481 tttgtccaaa ctcatcaatg tatcttatca tgtct
//
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